Bishwa Raj Sapkota
نویسندگان
چکیده
Differentiation of M. tuberculosis and M leprae by polymerase chain reaction (PCR), when acid-fast bacilli (AFB) were present in sputum from patients at Anandaban hospital, was carried out. Thirty sputum samples microscopy positive for AFB were collected and were subjected to culture. Bacterial DNA was extracted and PCR was performed using primers specific for Mycobacterium tuberculosis and Mycobacterium leprae DNA. Twenty samples were from patients with clinical TB and 10 from patients with clinical leprosy. Fifteen of the TB samples were positive in both TB PCR and culture, among the reminders four were TB PCR negative and one was positive for TB PCR. All TB samples were negative for leprosy PCR. Of the leprosy samples, five were TB PCR and culture positive, and negative for leprosy PCR. The remaining five samples were negative for both TB PCR and culture but positive in leprosy PCR. Five of ten clinical leprosy samples were positive for tuberculosis. This indicates that AFB in the sputum of leprosy patients might be M. tuberculosis or M. leprae. Thus PCR can be used for rapid differentiation of M. tuberculosis and M. leprae present in sputum where AFB microscopy is inconclusive.
منابع مشابه
A retrospective study of the effect of modified multi-drug therapy in Nepali leprosy patients following the development of adverse effects due to dapsone.
INTRODUCTION Dapsone Hypersensitivity Syndrome (DHS) occurs in approximately 2% of leprosy patients in Nepal. DHS and other adverse effects of dapsone lead to withdrawal of the drug. METHODS We reviewed the notes of patients who had dapsone withdrawn from their multi-drug therapy (MDT) following an adverse reaction to the drug between 1990 and 2007. RESULTS 105 patients were identified from...
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